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Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system
Marjeta Hofko1, Alexander Mischnik, Martin Kaase
1Department of Infectious Diseases, Medical Microbiology and Hygiene, Heidelberg University Hospital, Heidelberg, Germany.
Journal of Clinical Microbiology
|February 14, 2014
Abstract:
We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.

