Related Experiment Video
Updated: May 3, 2026

Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Generation of TALEN-mediated GRdim knock-in rats by homologous recombination
Verónica Ponce de León1, Anne-Marie Mérillat1, Laurent Tesson2
1Department of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland.
Abstract:
Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.
Related Concept Videos
In-vitro Mutagenesis
Homologous Recombination
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...

