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High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
Published on: January 21, 2015
Fourier transform infrared spectroscopy: a potential technique for noninvasive detection of spermatogenesis
Kambiz Gilany1, Roudabeh Sadat Moazeni Pouracil2, Mohammad Reza Sadeghi1
1Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Background:
The seminal plasma is an excellent source for noninvasive detection of spermatogenesis. The seminal plasma of normospermic and azoospermic men has been analyzed for detection of spermatogenesis.
Methods:
Optical spectroscopy (Attenuated Total Reflectance-Infrared spectroscopy (ATR-IR) and Fourier Transform infrared spectroscopy (FT-IR) has been used to analyze the seminal plasma and the metabolome of seminal plasma for detection of spermatogenesis.
Results:
The seminal plasma of normospermic and azoospermic men has been analyzed by ATR-IR. The results show that there is a pattern variation in the azoospermic men compared to normospermic men. However, the seminal plasma is too complex to show significant pattern variation. Therefore, the metabolome which is a subcomponent of the seminal plasma was analyzed. The seminal plasma metabolome of normospermic and azoospermic men has been analyzed by FT-IR. A significant pattern change was observed. The data combined with chemometrics analysis showed that significant changes are observed at metabolome level.
Conclusion:
We suggest that FT-IR has the potential as a diagnostic tool instead of testicular biopsy.
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