Related Experiment Video

Updated: May 3, 2026

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

9.9K

Exosome-formed synthetic microRNA-143 is transferred to osteosarcoma cells and inhibits their migration

Keisuke Shimbo1, Shigeru Miyaki2, Hiroyuki Ishitobi3

  • 1Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.

Biochemical and Biophysical Research Communications
|February 15, 2014
PubMed

Insights

Synthetic microRNAs (miRNAs) show anticancer promise. Exosomes effectively deliver miR-143 to osteosarcoma cells, inhibiting their migration, suggesting exosomes as a viable delivery system.

Area of Science:

  • Biotechnology
  • Cancer Research
  • Cell Biology

Background:

  • MicroRNAs (miRNAs) are investigated as anticancer agents.
  • Clinical use of miRNAs is hindered by ineffective tumor delivery systems.
  • Exosomes are key mediators of intercellular communication.

Purpose of the Study:

  • To investigate exosomes as a delivery system for synthetic microRNAs.
  • To evaluate the efficacy of exosome-delivered miR-143 in osteosarcoma cells.

Main Methods:

  • Synthetic miR-143 was introduced into cells.
  • Exosomes were collected after cells released them.
  • Exosome-formed miR-143 was delivered to osteosarcoma cells.
  • Cell migration was assessed.

Main Results:

  • Synthetic miR-143 was successfully encapsulated and released by exosomes.
  • Exosome-mediated delivery of miR-143 inhibited osteosarcoma cell migration.
  • While lipofection showed higher delivery efficiency, both methods similarly reduced cell migration.

Conclusions:

  • Exosomes can efficiently deliver synthetic miR-143 to target cells.
  • Exosome-formed miR-143 demonstrates functional anticancer activity by reducing cell migration.
  • Exosomes represent a promising and functional delivery platform for miRNA-based cancer therapy.
Keywords:
Delivery systemExosomeOsteosarcoma cellsmiR-143

More Related Videos

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

6.7K
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

10.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K