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Updated: May 3, 2026
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
Published on: March 14, 2019
Cytoplasmic polyhedrosis virus-induced differential gene expression in two silkworm strains of different
Kun Gao1, Xiang-Yuan Deng2, He-Ying Qian3
1College of Biotechnology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu, China; Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu, China.
Abstract:
Digital gene expression (DGE) was performed to investigate the gene expression profiles of 4008 and p50 silkworm strains at 48 h after oral infection with BmCPV. 3,668,437 clean tags were identified in the BmCPV-infected p50 silkworms and 3,540,790 clean tags in the control p50. By contrast, 4,498,263 clean tags were identified in the BmCPV-infected 4008 silkworms and 4,164,250 clean tags in the control 4008. A total of 691 differentially expressed genes were detected in the infected 4008 DGE library and 185 were detected in the infected p50 DGE library, respectively. The expression profiles identified some important differentially expressed genes involved in signal transduction, enzyme activity and apoptotic changes, some of which were verified using quantitative real-time PCR (qRT-PCR). These results provide important clues on the molecular mechanism of BmCPV invasion and resistance mechanism of silkworms against BmCPV infection.
Insights
This study reveals key gene expression changes in silkworms following Bombyx mori multiple nucleopolyhedrovirus (BmCPV) infection. Differential gene expression highlights pathways involved in silkworm resistance to viral invasion.
Area of Science:
- Entomology
- Molecular Biology
- Genomics
Background:
- Bombyx mori multiple nucleopolyhedrovirus (BmCPV) is a significant pathogen affecting silkworm production.
- Understanding silkworm-virus interactions is crucial for developing effective disease management strategies.
Purpose of the Study:
- To investigate the gene expression profiles of two silkworm strains (4008 and p50) after BmCPV infection.
- To identify differentially expressed genes (DEGs) associated with viral resistance mechanisms.
Main Methods:
- Digital Gene Expression (DGE) analysis was performed on BmCPV-infected and control silkworms (strains 4008 and p50).
- Quantitative real-time PCR (qRT-PCR) was used to validate the expression of selected genes.
Main Results:
- A substantial number of clean tags were identified in both infected and control groups across both silkworm strains.
- 691 DEGs were detected in the infected 4008 strain, and 185 DEGs were identified in the infected p50 strain.
- DEGs were primarily involved in signal transduction, enzyme activity, and apoptotic processes.
Conclusions:
- The study provides insights into the molecular mechanisms underlying BmCPV infection in silkworms.
- Identified DEGs offer potential targets for understanding and enhancing silkworm resistance to BmCPV.
