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Updated: May 3, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
Published on: February 18, 2022
Substantial expression of novel small open reading frames in Oryza sativa
Masanori Okamoto1, Mieko Higuchi-Takeuchi2, Minami Shimizu2
1Arid Land Research Center; Tottori University; Tottori, Japan; RIKEN Center for Sustainable Resource Science; Yokohama, Japan.
Abstract:
In our previous integrated study combining informatics and molecular biology analyses, we revealed that Arabidopsis small open reading frames (sORFs) predicted by computational analysis have biological functions in morphogenesis. Here, we report that sequences homologous to Arabidopsis sORFs are abundant in intergenic regions of the rice genome. These sequences represent a subset of non-protein-coding DNA, and some are transcribed into mRNA. These results indicate that many sORFs associated with morphogenesis are hidden in the genomes of crop species.
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