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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

Mechanically-gated Ion Channels

Mechanically-gated Ion Channels

Mechanically-gated Ion Channels

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

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Related Experiment Video

Updated: May 3, 2026

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Studying mechanosensitive ion channels with an automated patch clamp.

Maria Barthmes¹, Mac Donald F Jose, Jan Peter Birkner

¹Nanion Technologies GmbH, Gabrielenstr. 9, 80636, Munich, Germany.

European Biophysics Journal : EBJ

|February 15, 2014

Summary

Automated patch clamp systems now enable mechanosensitive ion channel (MSC) studies. This technique successfully patched liposomes and E. coli spheroplasts, yielding reliable data for high-throughput screening.

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Last Updated: May 3, 2026

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress

Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress

Published on: August 2, 2019

Area of Science:

Biophysics
Molecular Biology
Membrane Protein Research

Background:

Patch clamp electrophysiology is crucial for studying mechanosensitive ion channels (MSCs).
Conventional patch clamping is labor-intensive and operator-dependent, limiting throughput.
Existing automated patch clamp systems are not suitable for MSCs.

Purpose of the Study:

To report the activation and single channel analysis of a bacterial MSC using an automated patch clamp system.
To demonstrate the applicability of automated patch clamp systems to MSC research.
To enable high-throughput studies on MSCs.

Main Methods:

Utilized an automated patch clamp system for electrophysiological recordings.
Successfully patched giant unilamellar liposomes and giant Escherichia coli (E. coli) spheroplasts.
Performed activation and single channel analysis of a bacterial mechanosensitive ion channel.

Main Results:

The automated system enabled patching of both liposomes and E. coli spheroplasts.
Obtained tension sensitivity and channel kinetics data consistent with conventional patch clamp.
Demonstrated the feasibility of automated patch clamp for MSC analysis.

Conclusions:

Automated patch clamp systems can be effectively applied to study mechanosensitive ion channels.
This approach overcomes limitations of conventional methods, enabling higher throughput.
Findings facilitate fundamental research and drug screening for mechanosensitive ion channels.