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Updated: May 3, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
Published on: September 5, 2019
Martin Maška1, Vladimír Ulman2, David Svoboda2
1Center for Biomedical Image Analysis, Masaryk University, 602 00 Brno, Czech Republic, Cancer Imaging Laboratory, Oncology Division, Center for Applied Medical Research, University of Navarra, 31008 Pamplona, Spain, Biomedical Imaging Group Rotterdam, Erasmus University Medical Center, 3015 GE Rotterdam, The Netherlands, Fusion Technology and Systems Department, Compunetix Inc., Monroeville, PA 15146, USA, Biomedical Computer Vision Group, Department of Bioinformatics and Functional Genomics, University of Heidelberg, BIOQUANT, IPMB and DKFZ, 69120 Heidelberg, Germany, KTH Royal Institute of Technology, ACCESS Linnaeus Center, Department of Signal Processing, 100 44 Stockholm, Sweden, Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA, Division of Image Processing, Leiden University Medical Center, 2300 RC Leiden, The Netherlands, Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, 12801 Prague 2, Czech Republic and Biomedical Image Technologies, Universidad Politécnica de Madrid & CIBER BBN, 28040 Madrid, SpainCenter for Biomedical Image Analysis, Masaryk University, 602 00 Brno, Czech Republic, Cancer Imaging Laboratory, Oncology Division, Center for Applied Medical Research, University of Navarra, 31008 Pamplona, Spain, Biomedical Imaging Group Rotterdam, Erasmus University Medical Center, 3015 GE Rotterdam, The Netherlands, Fusion Technology and Systems Department, Compunetix Inc., Monroeville, PA 15146, USA, Biomedical Computer Vision Group, Department of Bioinformatics and Functional Genomics, University of Heidelberg, BIOQUANT, IPMB and DKFZ, 69120 Heidelberg, Germany, KTH Royal Institute of Technology, ACCESS Linnaeus Center, Department of Signal Processing, 100 44 Stockholm, Sweden, Baxter Laboratory for Stem Cell Biology, Department of Microbiology and
A new benchmark for evaluating cell tracking algorithms in microscopy was created. This framework objectively compares and ranks various methods using diverse datasets, aiding future research in biomedical imaging.
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