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Related Experiment Videos

Tenascin interferes with fibronectin action.

R Chiquet-Ehrismann1, P Kalla, C A Pearson

  • 1Friedrich Miescher Institut, Basel, Switzerland.

Cell
|May 6, 1988
PubMed
Summary
This summary is machine-generated.

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Tenascin inhibits cell spreading and attachment to substrates like fibronectin. Blocking tenascin

Area of Science:

  • Cell biology
  • Biochemistry
  • Extracellular matrix research

Background:

  • Cell attachment and spreading are crucial for tissue development and function.
  • The extracellular matrix (ECM) plays a vital role in regulating cell behavior.
  • Tenascin and fibronectin are key ECM proteins with distinct cellular interactions.

Purpose of the Study:

  • To investigate the role of tenascin in regulating fibroblast attachment and morphology.
  • To determine how tenascin influences cell interactions with other ECM components.
  • To identify the specific region of tenascin responsible for its inhibitory effects on cell attachment.

Main Methods:

  • Coating substrates with varying ratios of tenascin and fibronectin.
  • Observing primary chick and rat fibroblast attachment and morphology.

Related Experiment Videos

  • Assessing the effects of tenascin on integrin-mediated cell adhesion.
  • Utilizing monoclonal antibodies and Fab fragments against tenascin to block its activity.
  • Employing electron microscopy to localize antibody binding sites on tenascin.

    • Main Results:

    • Chick fibroblasts attached to tenascin but remained rounded, indicating inhibited spreading.
    • Tenascin inhibited integrin-mediated attachment to fibronectin, laminin, and GRGDS peptide.
    • Tenascin also inhibited rat fibroblast attachment to fibronectin but not laminin.
    • Monoclonal antibodies and Fab fragments against tenascin neutralized its inhibitory effects.
    • The epitope for these antibodies was localized to the terminal knob of tenascin's arms.

    Conclusions:

    • Tenascin acts as an inhibitor of cell spreading and attachment, particularly in conjunction with other ECM proteins.
    • The inhibitory function of tenascin is mediated by masking specific cell-binding sites.
    • The terminal knob region of tenascin contains a critical epitope involved in regulating cell adhesion.