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Updated: May 3, 2026
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
Systems-Level Lipid Analysis Methodologies for Qualitative and Quantitative Investigation of Lipid Signaling Events
Dayanjan S Wijesinghe1, Charles E Chalfant2
1Research and Development, Hunter Holmes McGuire Veterans' Affairs Medical Center , Richmond, Virginia. ; Department of Biochemistry and Molecular Biology, School of Medicine, Virginia Commonwealth University , Richmond, Virginia. ; Virginia Commonwealth University Reanimation Engineering Science Center (VCURES); Virginia Commonwealth University , Richmond, Virginia.
Objective:
Accumulating evidence implicates a prominent role for lipid signaling molecules in the regulation of wound healing. These lipids regulate hemostasis, onset and resolution of inflammation, migration and proliferation cells, angiogenesis, epithelialization, and remodeling of collagen. The objective of this overview is to demonstrate the applicability of systems level lipid analyses to identify and quantify lipid involved in events leading to wound healing.
Approach:
Current advances in liquid chromatography coupled to tandem mass spectrometry have provided the means for carrying out quantitative and qualitative analysis of lipids at a systems level. This emerging field is collectively referred to as lipidomics and its potential in wound healing research is largely ignored.
Results:
While comprehensive applications of lipidomics in wound healing are limited, studies carried out by the authors as well as others demonstrate distinct changes in the lipidome during the wound healing process.
Innovation:
Until recently, investigations into lipids were limited to the study of a few lipids at a time. Lipidomics approaches provide the capability to quantitatively and qualitatively assay almost the full complement of lipid signaling circuits at the same time. This allows obtaining a system level understanding of changes to the entire lipidome during the wound healing process.
Conclusion:
The technology provides promising approach to understanding new signaling pathways based on lipids involved in wound healing. The understanding gained from such studies has the potential for the development of novel lipid based treatment strategies to promote wound healing.
