Quantitative approach to track lipase producing Pseudomonas sp. S1 in nonsterilized solid state fermentation

Area of Science:

• Biotechnology
• Enzyme Technology
• Microbial Fermentation

Background:

• Lipase production is crucial for various industrial applications.
• Cost-effective enzyme production methods are in high demand.
• Agro-industrial waste offers a sustainable substrate for fermentation.

Purpose of the Study:

• To evaluate lipase production by Pseudomonas sp. S1 using nonsterile solid-state fermentation (NSSF) with agro-waste.
• To characterize the produced lipase for industrial applications.
• To establish a cost-effective method for lipase production.

Main Methods:

• Quantitative evaluation of Pseudomonas sp. S1 proliferation using ERIC-PCR during NSSF.
• Lipase production and activity assays under different fermentation conditions.
• Characterization of lipase stability at varying pH, temperature, and methanol concentrations.

Main Results:

• Pseudomonas sp. S1 produced 57.9 IU/g lipase in NSSF with olive oil cake.
• PCR-based enumeration showed bacterial proliferation from 7.6 × 10^4 CFU/g to 4.6 × 10^8 CFU/g, correlating with lipase activity.
• The crude lipase exhibited optimal activity at pH 7.0 and 50°C, with significant stability in methanol.

Conclusions:

• This study reports the first thermophilic, organic solvent-tolerant lipase production using agro-waste in NSSF.
• A positive correlation between Pseudomonas sp. S1 survival and lipase production in NSSF was established.
• The findings offer a cost-effective and industrially relevant approach to lipase production.