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Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: May 3, 2026

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

Published on: September 14, 2016

Expertise under the microscope: processing histopathological slides.

Thomas Jaarsma¹, Halszka Jarodzka, Marius Nap

¹Centre for Learning Sciences and Technologies (CELSTEC), Open Universiteit in The Netherlands, Heerlen, The Netherlands.

Medical Education

|February 18, 2014

Summary

This summary is machine-generated.

Expert and intermediate pathologists achieve similar diagnostic accuracy using different visual strategies. Novices struggle with histopathology slide analysis, highlighting training needs for all skill levels.

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Last Updated: May 3, 2026

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Published on: September 14, 2016

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Area of Science:

Pathology
Medical Education
Cognitive Science

Background:

Understanding expertise development in clinical pathology is crucial for effective training.
Developmental stages and cognitive characteristics of diagnosticians are not well-defined.

Purpose of the Study:

To investigate expertise-related differences in histopathological slide processing.
To combine eye-tracking and verbal data to analyze diagnostic strategies.

Main Methods:

Study involved clinical pathologists (experts), pathology residents (intermediates), and medical students (novices).
Participants diagnosed colon tissue images for 2 seconds while eye movements, diagnoses, and verbal explanations were recorded.
Eye movements were analyzed for temporal changes and relevant area processing; verbal data underwent content analysis.

Main Results:

Experts and intermediates demonstrated comparable diagnostic accuracy but employed distinct visual and cognitive strategies.
Experts focused on initial findings and broader abnormalities, while intermediates re-verified initial diagnoses.
Novices showed poor accuracy, with less attention to relevant areas and inconclusive explanations.

Conclusions:

Different visual and cognitive strategies can lead to equal diagnostic accuracy in intermediates and experts.
Training should emphasize distinguishing normal from abnormal tissue for novices.
Intermediates require training to identify subtle abnormalities, suggesting stage-specific educational designs and feedback.