Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A swab and wetting agent combination investigation for enhanced touch DNA recovery: A step towards standardisation.

International journal of legal medicine·2026
Same author

Acute Thermal Tolerance and Physiological Responses in Commercial and Native Red-Feathered Roosters Sharing the Same HSP70 Homozygous Genotype.

Animals : an open access journal from MDPI·2026
Same author

Using hierarchical Bayesian modelling to assess shedder test suitability.

Forensic science international. Genetics·2026
Same author

Does Pre- and Intraoperative Culture Negativity Affect the Outcome of Debridement, Antibiotics, and Implant Retention in Acute Periprosthetic Joint Infection: A Single-Center Retrospective Cohort Study.

The Journal of arthroplasty·2026
Same author

Differential impact of single interim spacer exchanges on survival outcomes in two-stage exchange arthroplasty for PJIs: a comparative analysis with minimum 5-year follow-up.

BMC musculoskeletal disorders·2026
Same author

Separating sperm from cell mixtures by using a bubble-based acoustofluidic filtration device for forensic DNA analysis.

Forensic science international. Genetics·2026

Related Experiment Video

Updated: May 3, 2026

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
07:34

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

3.3K

Establishing a DNA identification system for pigs (Sus scrofa) using a multiplex STR amplification.

Yu-Chih Lin1, Hsing-Mei Hsieh2, James Chun-I Lee3

  • 1Department of Graduate Institute of Biotechnology, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC; Taichung City Government Police Department, 588 Wenxin Road Section 2, Xitun District, Taichung City 40758, Taiwan, ROC.

Forensic Science International. Genetics
|February 18, 2014
PubMed
Summary

A new 14-plex PCR system using 13 tetra-nucleotide STRs and amelogenin was developed for pig forensic identification. This novel system accurately individualizes pigs, aids parentage testing, and supports breed and phylogenetic studies.

Keywords:
Parentage testingPig DNA identificationPig individualizationSTR multiplexSus scrofa

More Related Videos

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
10:16

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Published on: February 14, 2016

9.8K
A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
07:40

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

24.9K

Related Experiment Videos

Last Updated: May 3, 2026

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
07:34

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

3.3K
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
10:16

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Published on: February 14, 2016

9.8K
A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
07:40

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

24.9K

Area of Science:

  • Animal genetics
  • Forensic science
  • Molecular biology

Background:

  • Accurate genetic identification is crucial for forensic applications in livestock.
  • Existing methods may lack the discriminatory power for individual pig identification and breed analysis.

Purpose of the Study:

  • To develop and validate a novel multiplex PCR system for pig forensic identification.
  • To assess the genetic variation and discriminatory capacity of the system across different Taiwanese pig breeds.

Main Methods:

  • Developed a 14-plex PCR assay using 13 tetra-nucleotide STRs and the amelogenin marker.
  • Genotyped 341 pigs from 11 breeds in Taiwan, analyzing allele frequencies and genetic variation.
  • Validated the system's sensitivity, specificity, and utility in individualization, parentage testing, and phylogenetic analysis.

Main Results:

  • The 14-plex system demonstrated high discriminatory power, with combined match probability (CPm) of 1.31 E-11 and combined paternity exclusion (CPEtrio) of 0.9996.
  • Paternity testing (CPItrio) ranged from 4.012 E+4 to 4.332 E+6.
  • Phylogenetic analysis revealed distinct clustering of Asian and European breeds, with hybrids forming intermediate clades.

Conclusions:

  • The novel 14-plex PCR system is a powerful tool for pig individualization, parentage testing, breed assessment, and forensic applications.
  • The system exhibits high sensitivity, specificity, and robustness across various tissue types.
  • Phylogenetic insights into pig breed relationships were successfully obtained using this genetic marker system.