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A case study of SNPSTR efficiency in paternity testing with locus incompatibility
Yi Ye1, Haibo Luo2, Linchuan Liao3
1Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China; Department of Forensic Analytical Toxicology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
SNPSTR, combining STR and SNP markers, enhances paternity testing accuracy. This novel tool significantly increases paternity probability, proving valuable in forensic science applications.
Area of Science:
- Forensic genetics
- Molecular biology
Background:
- Traditional paternity testing relies on Short Tandem Repeat (STR) loci.
- Single Nucleotide Polymorphism (SNP) markers offer an alternative or supplementary approach.
- Combining STR and SNP data can potentially improve discriminatory power.
Observation:
- A novel SNPSTR marker was developed using a CSF1PO STR locus and five linked SNPs.
- The SNPSTR marker was evaluated in a paternity testing case with an inconsistency at the CSF1PO locus.
- 152 haplotypes from 76 individuals were analyzed using nested-AS-PCR, yielding 60 unique SNPSTR haplotypes.
Findings:
- The combined SNPSTR haplotype demonstrated higher discrimination power than STR or SNP haplotypes alone.
- The SNP component of SNPSTR was effective in differentiating fathers from uncles.
- Incorporating SNPSTR into parentage calculations increased the paternity probability to 99.998%.
Implications:
- SNPSTR offers a powerful tool for forensic science, particularly in complex paternity testing scenarios.
- This combined approach can resolve cases with ambiguous results from traditional STR analysis.
- SNPSTR has the potential to improve the reliability and efficiency of forensic genetic investigations.
