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Updated: May 3, 2026

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
Published on: October 2, 2018
Coffea arabica instant coffee--chemical view and immunomodulating properties
Peter Capek1, Ema Paulovičová2, Mária Matulová3
1Institute of Chemistry, Department of Glycomaterials, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
Abstract:
Results of chemical analyses and immunological studies of two Coffea arabica instant coffee powders obtained by freeze-dried (ICPf) and spray-dried (ICPs) procedures, and arabinogalactan-protein (AGP3) obtained from ICPf are presented. For instant coffee powders no significant differences have been found in carbohydrate (ICPf: 37%, ICPs: 38%) as well as in caffeine (ICPf: 3.0%, ICPs: 3.4%) contents. Their (1)H NMR spectra revealed differences in trigonelline and chlorogenic acids content and in a degree of AGP backbone substitution. Immunobiological tests of all samples (ICPf, ICPs, AGP2 and AGP3) revealed a significant immunostimulatory effect on induction of interleukin 2 and free radicals secretion by mice immunocytes. Moreover, tests revealed more pronounced effect of arabinogalactans AGP2 and AGP3 compared to instant coffee powders (ICPf and ICPs).
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