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Related Experiment Video

Updated: May 3, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
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Methods for performing lipidomics in white adipose tissue.

Lee D Roberts1, James A West1, Antonio Vidal-Puig2

  • 1MRC Human Nutrition Research, The Elsie Widdowson Laboratory, Cambridge, United Kingdom; Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom.

Methods in Enzymology
|February 18, 2014
PubMed
Summary

This study explores mass spectrometry (MS) techniques for analyzing lipid metabolism in white adipose tissue. It details methods for lipid extraction and profiling, crucial for understanding tissue function and inflammation.

Keywords:
EicosanoidsFatty acidsMass spectrometryPhospholipidsTriacylglycerides

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Area of Science:

  • Biochemistry
  • Metabolomics
  • Adipose Tissue Biology

Background:

  • Lipid metabolism is critical for white adipose tissue (WAT) function, involving triacylglyceride storage and free fatty acid release.
  • Lipid species are implicated in lipotoxicity and signaling during adipose tissue inflammation.
  • Understanding the lipid profile of WAT is essential for metabolic research.

Purpose of the Study:

  • To detail mass spectrometry (MS) methods for comprehensive lipid profiling in adipose tissue.
  • To provide established protocols for lipid extraction and fatty acid composition analysis.
  • To present advanced liquid chromatography-MS approaches for global lipid profiling and eicosanoid analysis in WAT.

Main Methods:

  • Lipid extraction using the Folch procedure.
  • Gas chromatography-mass spectrometry (GC-MS) for total fatty acid composition analysis.
  • Liquid chromatography-mass spectrometry (LC-MS) for profiling phospholipids, triacylglycerides, free fatty acids, and eicosanoids.

Main Results:

  • Established GC-MS method for quantifying total fatty acid composition in rodent and human adipose tissue.
  • Developed LC-MS methods for comprehensive profiling of major lipid classes and signaling molecules.
  • Demonstrated the utility of MS-based approaches for detailed adipose tissue lipidomics.

Conclusions:

  • Mass spectrometry provides powerful tools for dissecting complex lipid metabolism in white adipose tissue.
  • These methods enable in-depth analysis of lipid species relevant to metabolic health and disease.
  • The described techniques are valuable for research into obesity, insulin resistance, and type 2 diabetes.