Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multiple Antibiotic Allergy Evaluation Strategy (MAAES): A Comparative Effectiveness Study.

The journal of allergy and clinical immunology. In practice·2026

Same author

Reported safety events from ultrasound enhancing agents: a critical reappraisal.

Echo research and practice·2026

Same author

Interpretation of Extracorporeal Membrane Oxygenation as a Bridge to Heart Transplantation: Is This the Next Horizon?

Journal of cardiothoracic and vascular anesthesia·2026

Same author

Persistence of organic and inorganic gunshot residues on hands, forearms, and face of shooters 24 h after a high number of discharges.

Forensic science international·2026

Same author

Sovereign AI supercomputers: a global landscape review of unprecedented biomedical research infrastructure.

Frontiers in artificial intelligence·2026

Same author

Hepatitis at Home: Ambulatory management of severe immune checkpoint inhibitor related hepatitis.

QJM : monthly journal of the Association of Physicians·2026

Search research articles

Related Experiment Video

Updated: May 3, 2026

Study of a Dot-patterning Process on Flexible Materials using Impact Print-Type Hot Embossing Technology

Study of a Dot-patterning Process on Flexible Materials using Impact Print-Type Hot Embossing Technology

Published on: April 6, 2020

Evaluation of fingermark detection sequences on paper substrates.

Callie Marriott¹, Rebecca Lee², Zachary Wilkes²

¹National Centre for Forensic Studies, University of Canberra, Canberra, ACT 2601, Australia.

Forensic Science International

|February 18, 2014

Summary

The 1,2-indanedione-zinc chloride (IND-Zn) sequence followed by ninhydrin and physical developer (PD) is recommended for detecting latent fingermarks on paper. This method generally outperformed the 1,8-diazafluoren-9-one (DFO) sequence in trials.

Keywords:

1,2-Indanedione 1,8-Diazafluoren-9-one Fingerprints Nile red Ninhydrin Physical developer Porous surfaces

More Related Videos

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

Related Experiment Videos

Last Updated: May 3, 2026

Study of a Dot-patterning Process on Flexible Materials using Impact Print-Type Hot Embossing Technology

Study of a Dot-patterning Process on Flexible Materials using Impact Print-Type Hot Embossing Technology

Published on: April 6, 2020

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

Area of Science:

Forensic Science
Chemistry
Trace Evidence Analysis

Background:

The 1,2-indanedione-zinc chloride (IND-Zn) reagent is considered optimal for latent fingermark detection on paper.
Ninhydrin's effectiveness is limited when used sequentially after IND-Zn.
Recent research suggests the 1,8-diazafluoren-9-one (DFO) followed by ninhydrin sequence may yield more fingermarks than IND-Zn alone.

Purpose of the Study:

To evaluate two fingermark detection sequences on porous surfaces: IND-Zn followed by ninhydrin, physical developer (PD), and nile red; and DFO followed by ninhydrin, PD, and nile red.
To compare the effectiveness of these sequences across various donors and Australian paper substrates.
To assess the performance in a pseudo-operational trial using aged examination booklets under different climatic conditions.

Main Methods:

Comparative evaluation of two fingermark detection sequences: (1) IND-Zn/ninhydrin/PD/nile red and (2) DFO/ninhydrin/PD/nile red.
Testing on diverse latent fingermark donors and common Australian paper types.
Pseudo-operational trial on 5-year-old university examination booklets across two distinct climate locations (Sydney and Canberra).

Main Results:

Negligible performance difference between the two sequences across paper types and timeframes in donor studies.
IND-Zn generally produced higher quality fingermarks than DFO, but ninhydrin enhanced DFO marks more effectively.
The IND-Zn sequence outperformed the DFO sequence in pseudo-operational trials; nile red showed questionable value at the end of either sequence.

Conclusions:

The IND-Zn followed by ninhydrin and PD sequence is recommended for processing common paper substrates.
The effectiveness of the IND-Zn sequence is supported by both donor studies and pseudo-operational trials.
The addition of nile red at the end of the tested sequences provided no additional benefit for fingermark development.