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Updated: May 3, 2026

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
Published on: May 16, 2020
Competing aggregation pathways for monoclonal antibodies
Haixia Wu1, Rachel Kroe-Barrett2, Sanjaya Singh2
1Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19711, USA; Department of Biotherapeutics, Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, CT 06877, USA.
Abstract:
Aggregation is mediated by local unfolding to allow aggregation "hot spot(s)" to become solvent exposed and available to associate with a hot spot on another partially unfolded protein. Historically, the unfolding of either the crystallizable fragment (Fc) or the antigen binding fragment (Fab) regions of a given monoclonal antibody (MAb) has been implicated in aggregation, with differing results across different proteins. The present work focuses on separately quantifying the aggregation kinetics of isolated Fc, isolated Fab, and intact MAb as a function of pH under accelerated (high temperature) conditions. The results show that both Fab and Fc are aggregation prone and compete within the same MAb.
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