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Identification of putative endogenous proviral templates for progenitor mink cell focus-forming (MCF) MuLV-related
H Amanuma1, F Laigret, M Nishi
1Laboratory of Gene Technology and Safety, Institute of Physical and Chemical Research, Ibaraki, Japan.
Abstract:
Murine leukemia virus (MuLV)-related RNAs exhibiting different env deletions are believed to participate in the generation of leukemogenic mink cell focus-forming (MCF) viruses. We have cloned an endogenous MuLV provirus from AKR/J mouse DNA, designated as A-2, which may serve as template for the env-deleted E2 MuLV RNA, expressed in GIX+ mice (D.E. Levy et al., J. Virol. 56, 691-700 (1985]. We have also isolated an endogenous MCF-related DNA, A-1, which shared close sequence homology with the 7.2-kb RNA expressed in AKR mice (F. Laigret et al., J. Virol. 62, 376-386 (1988] and sustained an identical env deletion. The data indicate that putative precursor MCF-related RNAs are transcribed from a heterogenous family of env-deleted endogenous MuLV DNAs.
Insights
Endogenous murine leukemia virus (MuLV) DNAs with env deletions may generate leukemogenic mink cell focus-forming (MCF) viruses. Researchers identified specific MuLV and MCF-related DNA sequences in AKR/J mice, supporting this hypothesis.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Murine leukemia virus (MuLV)-related RNAs with env deletions are implicated in forming leukemogenic mink cell focus-forming (MCF) viruses.
- Endogenous MuLV proviruses are present in mouse DNA and can serve as templates for viral RNA.
- Previous studies have identified specific RNAs and their association with viral oncogenesis.
Purpose of the Study:
- To clone and characterize endogenous MuLV and MCF-related DNA sequences from AKR/J mouse DNA.
- To investigate the potential role of these endogenous sequences as templates for env-deleted RNAs.
- To understand the origins of leukemogenic MCF viruses.
Main Methods:
- Cloning of endogenous MuLV provirus (A-2) from AKR/J mouse DNA.
- Isolation of an endogenous MCF-related DNA (A-1) from AKR/J mouse DNA.
- Sequence homology analysis between isolated DNA and known viral RNAs.
Main Results:
- An endogenous MuLV provirus, A-2, was cloned and identified as a potential template for env-deleted E2 MuLV RNA.
- An endogenous MCF-related DNA, A-1, was isolated and found to share sequence homology with a 7.2-kb RNA expressed in AKR mice, possessing an identical env deletion.
- The findings suggest that env-deleted endogenous MuLV DNAs form a heterogeneous family that serves as precursors for MCF-related RNAs.
Conclusions:
- Endogenous MuLV DNAs with env deletions are likely precursors for leukemogenic MCF viruses.
- A heterogeneous family of env-deleted endogenous MuLV DNAs gives rise to MCF-related RNAs.
- This study provides insights into the genetic basis of MCF virus generation in mice.