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Updated: May 3, 2026

Surface Passivation for Single-molecule Protein Studies

Surface Passivation for Single-molecule Protein Studies

Published on: April 24, 2014

Process for protein PEGylation.

David Pfister¹, Massimo Morbidelli¹

¹Institute for Chemical and Bioengineering, ETH, Wolfgang-Pauli-Str. 10, 8093 Zurich, Switzerland.

Journal of Controlled Release : Official Journal of the Controlled Release Society

|February 18, 2014

Summary

This summary is machine-generated.

PEGylation enhances drug delivery by increasing protein persistence in the body. Optimizing PEGylation reaction engineering and purification is key for cost-effective, industrial-scale therapeutic protein production.

Keywords:

Bioengineering Drug delivery PEGylation Reaction engineering Separation

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Area of Science:

Bioconjugation Chemistry
Drug Delivery Systems
Process Engineering

Background:

PEGylation is a crucial technique in drug delivery, offering diverse conjugation chemistry and polymer structures.
It enhances therapeutic protein persistence in vivo without compromising activity.
High production costs of therapeutic proteins necessitate optimization of PEGylation processes.

Purpose of the Study:

To critically review the state-of-the-art in PEGylation reaction engineering and purification.
To focus on developing industrial-scale processes meeting market quality and cost requirements.
To explore the integration of continuous reaction and separation steps.

Main Methods:

Review of current literature on PEGylation reaction engineering.
Analysis of purification strategies for PEGylated proteins.
Evaluation of continuous processing and integrated reaction-separation systems.

Main Results:

Identified key challenges and advancements in PEGylation reaction engineering for industrial scale.
Highlighted optimized purification techniques to reduce costs and maintain protein activity.
Demonstrated the potential of integrated continuous processes for efficient manufacturing.

Conclusions:

Optimized PEGylation reaction engineering and purification are essential for cost-effective therapeutic protein production.
Continuous processing offers a promising avenue for scalable and efficient PEGylation.
Further development in these areas will facilitate market access for advanced biologic drugs.