Related Experiment Video

Updated: May 3, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.8K

Phenotypic and genotypic characteristics of Arcanobacterium haemolyticum isolated from clinical samples in a Danish

Osama Sammra1, Alice Friis-Møller, Anna Balbutskaya

  • 1Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392, Gießen, Germany.

Folia Microbiologica
|February 18, 2014
PubMed

Insights

This study identifies six Arcanobacterium haemolyticum strains using phenotypic and genotypic methods, including MALDI-TOF MS. Virulence gene analysis reveals varying profiles, aiding in the characterization of this increasingly important bacterial species.

Area of Science:

  • Medical Microbiology
  • Bacterial Pathogenesis

Background:

  • Arcanobacterium haemolyticum is a bacterial species of increasing clinical importance.
  • Accurate identification and characterization are crucial for understanding its pathogenicity.

Purpose of the Study:

  • To phenotypically and genotypically characterize Arcanobacterium haemolyticum strains isolated from human patients.
  • To investigate the presence and distribution of key virulence genes in these strains.

Main Methods:

  • Phenotypic identification including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
  • Genotypic identification via 16S rDNA and gap gene sequencing, and 16S-23S rDNA intergenic spacer region amplification.
  • Detection of seven potential virulence genes (arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, collagen binding protein, neuraminidase A, neuraminidase H).

Main Results:

  • Six Arcanobacterium haemolyticum strains were successfully identified from Danish hospitals.
  • Genotypic analysis confirmed phenotypic identification.
  • Virulence gene analysis showed varying presence, with strains harboring between four and seven genes.

Conclusions:

  • The combined phenotypic and genotypic approaches provide reliable methods for identifying and characterizing Arcanobacterium haemolyticum.
  • Understanding the virulence gene profiles is essential for assessing the pathogenic potential of clinical isolates.
  • This study contributes to the growing knowledge of Arcanobacterium haemolyticum as a significant human pathogen.

More Related Videos

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.6K
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium12:18

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

24.2K

Related Concept Videos

Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
854
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
90
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
2.3K