Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Conversion02:08

Gene Conversion

9.2K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.2K
Crossing Over01:34

Crossing Over

128.6K
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
128.6K
Crossing Over01:30

Crossing Over

6.3K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
6.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.7K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

16.3K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
16.3K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.1K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A proof-of-concept automated method for accurate skin dosimetry: correcting overestimated surface dose measurements.

Physics in medicine and biology·2026
Same author

Chemoport-related right innominate vein stenosis in patients with colorectal cancer: A retrospective risk factor analysis.

PloS one·2026
Same author

Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury.

Materials today. Bio·2026
Same author

Reply Re: "A Safer and Easier Method to Open the Orbital Septum: Septal Ballooning".

Ophthalmic plastic and reconstructive surgery·2026
Same author

Reply Re: "A Safer and Easier Method to Open the Orbital Septum: Septal Ballooning".

Ophthalmic plastic and reconstructive surgery·2026
Same author

Announcement: Journal of Structural Biology: Paper of the year.

Journal of structural biology·2025

Related Experiment Video

Updated: May 3, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

10.9K

Ternary and senary representations using DNA double-crossover tiles.

Byeonghoon Kim1, Soojin Jo, Junyoung Son

  • 1Department of Physics and Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746, Korea.

Nanotechnology
|February 18, 2014
PubMed
Summary

Researchers enhanced DNA double-crossover (DX) tile information capacity beyond binary. They achieved ternary and senary representations using DNA hairpins, verified by atomic force microscopy.

More Related Videos

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

6.2K
Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

933

Related Experiment Videos

Last Updated: May 3, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

10.9K
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

6.2K
Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

933

Area of Science:

  • Nanotechnology
  • Biochemistry
  • Materials Science

Background:

  • DNA nanotechnology utilizes DNA structures for information storage and computation.
  • Current DNA data storage often relies on binary representations, limiting information density.
  • DNA double-crossover (DX) tiles are versatile building blocks in DNA nanotechnology.

Purpose of the Study:

  • To increase the information capacity of DNA double-crossover (DX) tiles.
  • To explore higher base representations beyond binary for DNA data storage.
  • To investigate the structural and mechanical properties of these advanced DNA nanostructures.

Main Methods:

  • Designing and synthesizing DNA DX tiles with controlled DNA hairpin lengths and positions.
  • Utilizing atomic force microscopy (AFM) for structural verification of the DNA nanostructures.
  • Performing normal mode analysis to assess the mechanical characteristics of the modified DX tiles.

Main Results:

  • Successfully implemented ternary (base-3) and senary (base-6) digit representations in DNA DX tiles.
  • Demonstrated that controlling DNA hairpin features enables higher-order base encoding.
  • Characterized the mechanical properties of the DNA nanostructures, providing insights into their stability.

Conclusions:

  • The information capacity of DNA DX tiles can be significantly enhanced by moving beyond binary encoding.
  • DNA nanotechnology offers a promising platform for high-density data storage solutions.
  • The developed method provides a foundation for more complex DNA-based information systems.