Magnetic properties of ferritin and akaganeite nanoparticles in aqueous suspension

We have studied the magnetically induced optical birefringence Δn of horse spleen ferritin (HSF) and aqueous suspensions of several different-sized iron oxyhydroxide nanoparticles coated with different polysaccharides mimicking ferritin. The structure and dimensions of the akaganeite mineral core were characterized by XRD and TEM, respectively. The stability of the suspensions in the measurement temperature range from 278 to 358 K was confirmed by UV-Vis absorption spectroscopy. The values of optical polarizability anisotropy Δα, magnetic susceptibility anisotropy Δχ, and permanent magnetic dipole moment μ_m of the akaganeite nanoparticles have been estimated on the basis of the temperature dependence of the Cotton-Mouton (C-M) constant. The magnetic birefringence of Fe-sucrose has been described tentatively by different types of Langevin function allowing another estimation of Δχ and μ_m. The obtained permanent magnetic dipole moment μ_m of the studied akaganeite nanoparticles proves small and comparable to that of HSF. The value of μ_m is found to increase with decreasing nanoparticle diameter. Observed in a range spanning more than five orders of magnitude, the linear relation between the C-M constant and the iron concentration provides a basis for possible analytical application of the C-M effect in biomedicine. The established relation between the C-M constant and the nanoparticle diameter confirms that the dominant contribution to the measured magnetic birefringence comes from the magnetic susceptibility anisotropy Δχ. A comparison of the C-M constants of the studied akaganeite nanoparticles with the data obtained for HSF provides evidence that the ferritin core behaves as a non-Euclidian solid.