Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pesticides derived from egg yolks modulate cellular metabolic activity and proliferation, and increase steroidogenesis and oxidative stress in primary pheasant ovarian cells.

Environmental toxicology and pharmacology·2026
Same author

Carbendazim detection in human follicular fluid: Impact on human granulosa cell function (KGNs and primary cells) and mouse ovarian physiology.

Environmental toxicology and pharmacology·2026
Same author

Evaluation of the study carried out by ANSES on the effectiveness of sanitary shucking of queen scallops (<i>Aequipecten opercularis</i>) contaminated by okadaic acid toxins.

EFSA journal. European Food Safety Authority·2026
Same author

Mitochondrial fusion induced by Mdivi-1 and leflunomide alters cell proliferation and viability, steroidogenesis and oxidative stress in chicken ovarian cells.

Poultry science·2026
Same author

Molecular Dynamics and Quantum Chemical Insights into the Molecular Basis of Differential Toxicity of the Mycotoxin Deoxynivalenol (DON) and Its Microbial Derivatives DOM‑1 and 3-<i>epi</i>-DON.

ACS omega·2026
Same author

Biodiversity of Aspergillus section Flavi species isolated along the peanut paste production chain in Côte d'Ivoire.

International journal of food microbiology·2026

Related Experiment Video

Updated: May 3, 2026

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

6.2K

Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing.

Núria Mach1, Mustapha Berri2, Diane Esquerré3

  • 1UMR1313 Génétique Animale et Biologie Intégrative, INRA, Jouy-en-Josas, France ; UMR1313 Génétique Animale et Biologie Intégrative, AgroParisTech, Jouy-en-Josas, France ; DSV/iRCM/SREIT/LREG, CEA, Jouy-en-Josas, France ; UMR1319 MICALIS, INRA, Jouy-en-Josas, France ; UMR1319 MICALIS, AgroParisTech, Jouy-en-Josas, France.

Plos One
|February 18, 2014
PubMed
Summary

This study mapped gene expression in pig small intestine tissues. Results reveal distinct gene profiles for duodenum, jejunum, and ileum, highlighting tissue-specific biological processes.

More Related Videos

Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut
09:13

Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut

Published on: January 31, 2025

1.2K
Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
07:34

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

3.3K

Related Experiment Videos

Last Updated: May 3, 2026

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

6.2K
Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut
09:13

Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut

Published on: January 31, 2025

1.2K
Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
07:34

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

3.3K

Area of Science:

  • Genomics
  • Animal Science
  • Molecular Biology

Background:

  • The small intestine exhibits regional specialization crucial for nutrient absorption and immune surveillance.
  • Understanding tissue-specific gene expression is vital for deciphering intestinal function and disease.

Purpose of the Study:

  • To analyze and compare gene expression profiles across the duodenum, jejunum, and ileum, including ileal Peyer's patches, in healthy young pigs.
  • To establish baseline gene expression patterns for different small intestinal tissues.

Main Methods:

  • Transcriptome sequencing (RNA-Seq) was employed to analyze gene expression in four healthy pigs.
  • Multidimensional scaling was used to assess sample clustering based on tissue type.
  • Differential gene expression analysis was performed between various intestinal segments and Peyer's patches.

Main Results:

  • Gene expression profiles clustered by tissue type, confirming distinct molecular identities for duodenum, jejunum, and ileum.
  • Significant numbers of differentially expressed genes were identified between all compared small intestinal tissues.
  • Functional analysis indicated involvement in cell development, signaling, growth, and maintenance, with immune pathways enriched in Peyer's patches.

Conclusions:

  • The small intestine possesses distinct local specificities in gene expression related to tissue turnover and function.
  • Ileal Peyer's patches show an enrichment of enteric innate immune pathways, consistent with their role in immunity.
  • This gene expression map provides a reference for healthy states, aiding future studies on intestinal dysbiosis and pathology.