Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Study on a new chromogenic substrate for the prothrombin time determination.

A Girolami1, A Santarossa, S Martinelli

  • 1Institute of Medical Semeiotics, Medical School, University of Padua, Italy.

Folia Haematologica (Leipzig, Germany : 1928)
|January 1, 1987
PubMed
Summary

This study shows that chromogenic substrates can be reliably used for prothrombin time determination, correlating well with standard methods. While generally accurate, some prothrombin complex defect heterozygotes may require further investigation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antiplatelet glycoprotein autoantibodies in patients with autoimmune diseases with and without thrombocytopenia.

Journal of clinical immunology·1996
Same author

Unexpected low prevalence of FVIII gene inversion in severe hemophilia A patients from north-eastern Italy.

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis·1996
Same author

Pitfalls of protein C assays in patients with activated protein C resistance.

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis·1996
Same author

Compound heterozygous protein C deficiency resulting in the presence of only the beta-form of protein C in plasma.

Blood·1996
Same author

Inhibitory effect of prostacyclin and nitroprusside on type IIB von Willebrand factor-promoted platelet activation.

Thrombosis and haemostasis·1996
Same author

Homozygous patients with APC resistance may remain paucisymptomatic or asymptomatic during oral contraception.

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis·1996

Area of Science:

  • Clinical Chemistry
  • Hematology
  • Biochemistry

Background:

  • Prothrombin time (PT) is a critical measure of the extrinsic pathway of coagulation.
  • Traditional PT assays rely on visual clot detection, limiting automation and standardization.
  • Chromogenic substrates offer a spectrophotometric alternative for enzyme activity measurement.

Purpose of the Study:

  • To evaluate the utility of a chromogenic substrate assay (Chromoquick) for prothrombin time determination.
  • To compare the performance of the chromogenic assay against standard PT methods using different thromboplastins.

Main Methods:

  • Investigated normal subjects and various patient groups including liver disease, anticoagulant/heparin therapy, and prothrombin complex defects.
  • Assayed prothrombin time using the chromogenic reagent (Chromoquick) and two standard methods (Thromborel, Simplastin).

Related Experiment Videos

  • Correlated results between the chromogenic and standard prothrombin time assays.

    • Main Results:

    • Established normal ranges: 18-23 s for Chromoquick and 13.5-15.5 s for standard methods.
    • Demonstrated significant positive correlations (r = +0.505 to +0.947, p < 0.05 to < 0.001) between chromogenic and standard PT.
    • Observed that some heterozygotes for prothrombin complex defects were not unequivocally detected by the chromogenic assay.

    Conclusions:

    • Chromogenic substrate assays are a viable option for prothrombin time determination in laboratory practice.
    • The method offers potential advantages in standardization and automation.
    • Cost and potential limitations in detecting certain heterozygote factor deficiencies are considerations.