Antibacterial multilayer films fabricated by layer-by-layer immobilizing lysozyme and gold nanoparticles on
Bin Zhou1, Yan Li1, Hongbing Deng2
1College of Food Science and technology, Huazhong Agriculture University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
Abstract:
Negatively charged gold nanoparticles (GNP) and positively charged lysozyme (Lys) were alternately deposited on negatively charged cellulose mats via layer-by-layer (LBL) self-assembly technique. The fabricated multilayer films were characterized by energy-dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectra (FT-IR), and wide-angle X-ray diffraction (XRD). Morphology of the LBL film coated mats was observed by scanning electron microscopy (SEM). Thermal degradation properties were investigated by differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA). Additionally, the result of microbial inhibition assay indicated that the composite nanofibrous mats had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus, which could be used for antimicrobial packing, tissue engineering, wound dressing, etc.
