Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of diafiltration media and filtration modes on fouling and performance in skim milk microfiltration: A comparative study.

Journal of dairy science·2024

Same author

<i>In vitro</i> gastric digestion of polysaccharides in mixed dispersions: Evaluating the contribution of human salivary α-amylase on starch molecular breakdown.

Current research in food science·2024

Same author

Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions.

JDS communications·2022

Same author

Variation of in vitro digestibility of pea protein powder dispersions from commercially available sources.

Food chemistry·2022

Same author

Cellular lipids and protein alteration during biodegradation of expanded polystyrene by mealworm larvae under different feeding conditions.

Chemosphere·2022

Same author

A conceptual model of nitrogen dynamics for the Great Barrier Reef catchments.

Marine pollution bulletin·2021

Search research articles

Related Experiment Video

Updated: May 3, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

Modulation of immune function by milk fat globule membrane isolates.

R Zanabria¹, A M Tellez¹, M Griffiths¹

¹Department of Food Science, University of Guelph, Guelph, ON, N1G 2W5 Canada.

Journal of Dairy Science

|February 19, 2014

Summary

Milk fat globule membrane (MFGM) isolate suppressed immune cell activation without affecting proliferation or causing cell death. MFGM demonstrated potential as a functional ingredient modulating immune responses.

Keywords:

antiinflammatory bioactivity immunomodulation milk fat globule membrane

More Related Videos

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Published on: May 8, 2016

Related Experiment Videos

Last Updated: May 3, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Published on: May 8, 2016

Area of Science:

Immunology
Nutritional Science
Biochemistry

Background:

The immunomodulatory effects of minor milk components, specifically milk fat globule membrane (MFGM), are not fully elucidated.
Understanding MFGM's impact on mammalian immune function is crucial for its application in functional foods.

Purpose of the Study:

To investigate the in vitro immunomodulatory capacity of a milk fat globule membrane (MFGM) isolate.
To assess MFGM's effects on murine splenocyte proliferation, apoptosis, and cytokine production.

Main Methods:

Splenocyte proliferation assays were conducted.
Flow cytometry was used to analyze apoptosis.
Cytokine production (IFN-γ, TNF-α, IL-4) was measured.
Cells were stimulated with polyclonal activators like concanavalin A and lipopolysaccharide.

Main Results:

MFGM isolate did not affect basal splenocyte proliferation but suppressed it in the presence of polyclonal activators.
Flow cytometry results ruled out apoptosis as the mechanism for immune modulation.
MFGM significantly decreased the production of IFN-γ and TNF-α, indicating suppression of splenocyte activation.
The effect on IL-4 production was less pronounced compared to IFN-γ and TNF-α.
MFGM exhibited differential activity depending on the stimulant used (concanavalin A vs. lipopolysaccharide).

Conclusions:

Milk fat globule membrane (MFGM) isolate possesses immunomodulatory properties.
MFGM suppresses splenocyte activation, potentially through mechanisms other than apoptosis.
MFGM extracts show promise as bioactive functional ingredients for modulating immune responses.