Related Experiment Video

Updated: May 3, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

9.1K

Short communication: prevalence and antibiotic resistance of Staphylococcus aureus isolated from bovine clinical

Hossein Jamali1, Behrad Radmehr2, Salmah Ismail1

  • 1Biohealth Science Program, Institute of Biological Science, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.

Journal of Dairy Science
|February 19, 2014
PubMed

Insights

This study investigated Staphylococcus aureus in Iranian dairy cows, finding a 20.1% prevalence and significant antibiotic resistance. Key resistance genes like blaZ, tetM, and ermC were identified, informing control strategies for bovine mastitis.

Area of Science:

  • Veterinary Microbiology
  • Bovine Health
  • Antimicrobial Resistance

Background:

  • Bovine clinical mastitis poses a significant threat to dairy herds worldwide.
  • Staphylococcus aureus is a primary causative agent of mastitis, leading to economic losses.
  • Understanding antibiotic resistance patterns is crucial for effective treatment and control.

Purpose of the Study:

  • To determine the prevalence of Staphylococcus aureus in bovine clinical mastitis cases.
  • To identify antibiotic resistance profiles and specific resistance genes in isolated strains.
  • To provide data for developing targeted control programs in Varamin, Iran.

Main Methods:

  • Isolation and identification of Staphylococcus aureus from milk samples using standard microbiological techniques and API Staph system.
  • Detection of antibiotic resistance genes (blaZ, tetM, ermC) via Polymerase Chain Reaction (PCR).
  • Antimicrobial susceptibility testing using the disk diffusion method.

Main Results:

  • Staphylococcus aureus was isolated from 20.1% of bovine clinical mastitis samples.
  • Methicillin-resistant Staphylococcus aureus (MRSA) was found in 11.6% of isolates.
  • High resistance rates were observed for penicillin G (86%) and tetracycline (76.7%).
  • Prevalent resistance genes included blaZ (86%), tetM (39.5%), and ermC (39.5%).

Conclusions:

  • Staphylococcus aureus is a common cause of mastitis in the studied region.
  • Significant levels of antibiotic resistance, including MRSA, are present.
  • The identified resistance genes highlight the need for prudent antibiotic use and improved herd management practices.
Keywords:
Staphylococcus aureusantibiotic susceptibilityclinical mastitisresistance gene

More Related Videos

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

24.3K
Development and Assessment of Intracellular Infection Models for Staphylococcus aureus08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

1.3K

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
90
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
219
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
143
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
2.0K
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
2.3K
Antibiotic Selection00:57

Antibiotic Selection

Overview
48.9K