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Type-specific monoclonal antibodies produced to proteins of Murray Valley encephalitis virus
R A Hall1, G W Burgess, B H Kay
1Queensland Institute of Medical Research, Herston, Australia.
Immunology and Cell Biology
|February 1, 1988
Abstract:
Two monoclonal antibodies have been produced to the flavivirus, Murray Valley encephalitis (MVE). Immunofluorescent antibody and ELISA tests revealed that one antibody was specific for MVE while the other cross-reacted weakly with Alfuy and Kunjin viruses. These antibodies have neither haemagglutination inhibition nor neutralising activity. One of the monoclonal antibodies reacted with the envelope glycoprotein (E) and a 45,000 MW protein, while the other reacted with a protein of 23,000 daltons. Competitive binding assays confirmed that these antibodies reacted with unrelated epitopes.