Related Experiment Video
Updated: May 3, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
High bioethanol titre from Manihot glaziovii through fed-batch simultaneous saccharification and fermentation in
Anselm P Moshi1, Carla F Crespo2, Malik Badshah2
1Division of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden; Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, Uvumbuzi Road, Mwalimu J.K. Nyerere Mlimani Campus, University of Dar-es Salaam, P.O. Box 35179, Dar-es Salaam, Tanzania.
Abstract:
A process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (FB-SSF) of wild, non-edible cassava Manihot glaziovii. FB-SSF allowed fermentation of up to 390g/L of starch-derived glucose achieving high bioethanol concentration of up to 190g/L (24% v/v) with yields of around 94% of the theoretical value. The wild cassava M. glaziovii starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, Termamyl® and AMG®). The Automatic Gas Potential Test System (AMPTS) was adapted to yeast ethanol fermentation and demonstrated to be an accurate, reliable and flexible device for studying the kinetics of yeast in SSF and FB-SSF. The bioethanol derived stoichiometrically from the CO2 registered in the AMPTS software correlated positively with samples analysed by HPLC (R(2)=0.99).
More Related Videos
Related Concept Videos
Bioreactor Controls-III
Production of Organic Acids
Microbial Fermentation
Microbes in Beverage Production

