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Updated: May 3, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Genetic variation among quince (Cydonia oblonga Mill.) genotypes sampled from the Coruh valley in Turkey
E Orhan1, G Nardemir2, G Agar2
1Department of Agricultural Biotechnology, Faculty of Agriculture, Erzurum, Turkey.
Abstract:
Turkey has very rich quince genetic resources, and the country currently dominates world quince production. In particular, the northeastern part of the country has notable Cydonia oblonga Mill. germplasm. Authenticating the identity of germplasm resources of C. oblonga Mill. would be of great value for breeding practices. In the present study, genetic variations of 14 C. oblonga Mill. genotypes sampled from the Coruh valley of Turkey were investigated. Ten random primers generated 53 DNA markers. The highest polymorphism ratio was observed in the OPA07 primer (86%), while the lowest was observed in OPA03 (29%). The percentage of polymorphic bands was 51%, which demonstrated the efficiency of the primers used. The similarity matrix revealed that the similarity among genotypes ranged between 0.42 and 0.96. The identified random amplified polymorphic DNA markers enabled clear discrimination among all genotypes considered.
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