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Updated: Aug 11, 2026
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
Published on: April 9, 2019
Shared and unique determinants on human and murine intestinal epithelium as established by monoclonal antibodies
1Department of Medicine, Duke University Medical Center, Durham, NC 27710.
To characterize macromolecules from intestinal epithelium known to be reactive with human disease sera, monoclonal antibodies (MAbs) were elicited to determine cell localization, organ-specificity and cross-reactivity of the immunodominant determinants. Eight MAbs, reactive with murine epithelial cell-associated components by enzyme-linked immunosorbent assay, immunoblotting and immunofluorescence, demonstrated binding by the same techniques to constituents in determinant-bearing epithelial macromolecules were glycoproteins of approximately 180,000 (human and 199,000 (murine) mol. wt. Pre-incubation of sera from patients with chronic inflammatory bowel disease (but not normal human sera or control protein) inhibited binding of the 199 K macromolecule by three MAbs elicited and characterized in this study.
To characterize macromolecules from intestinal epithelium known to be reactive with human disease sera, monoclonal antibodies (MAbs) were elicited to determine cell localization, organ-specificity and cross-reactivity of the immunodominant determinants. Eight MAbs, reactive with murine epithelial cell-associated components by enzyme-linked immunosorbent assay, immunoblotting and immunofluorescence, demonstrated binding by the same techniques to constituents in determinant-bearing epithelial macromolecules were glycoproteins of approximately 180,000 (human and 199,000 (murine) mol. wt. Pre-incubation of sera from patients with chronic inflammatory bowel disease (but not normal human sera or control protein) inhibited binding of the 199 K macromolecule by three MAbs elicited and characterized in this study.
