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[Induction of interferon-specific enzymes in cultures of cells sensitive and resistant to interferon]
Abstract:
Induction of 2'-5'-oligoadenylatesynthetase (2-5A synthetase) by interferons and theophylline by means of activation of cAMP-system in interferon susceptible and resistant cell lines were studied. In interferon resistant cell lines the basal activity of 2-5A synthetase exceeded the level of the same enzyme in interferon susceptible cell lines. Activity of 2-5A synthetase is increased in interferon susceptible cell lines by interferon treatment, but the activity of the enzyme is not altered in interferon resistant cell lines. Among the studied cell lines the induction of 2-5A synthetase by theophylline was possible only in L929 cell line. The common mechanism for the absence of 2-5A synthetase induction by interferon and theophylline in interferon resistant cells is discussed.
Insights
Interferon resistance in cells correlates with higher basal 2-5A synthetase activity. Interferon treatment boosts 2-5A synthetase in susceptible cells but not resistant ones, suggesting a common resistance mechanism.
Area of Science:
- Molecular Biology
- Cell Biology
- Immunology
Background:
- Interferons (IFNs) induce antiviral states via 2-5A synthetase.
- IFN resistance can alter cellular responses to antiviral stimuli.
- The role of cAMP in 2-5A synthetase induction is not fully understood.
Purpose of the Study:
- To investigate 2-5A synthetase induction by IFNs and theophylline in IFN-susceptible and resistant cell lines.
- To explore the involvement of the cAMP system in these induction pathways.
- To identify potential common mechanisms underlying IFN and theophylline resistance.
Main Methods:
- Cell culture of IFN-susceptible and resistant lines.
- Measurement of 2-5A synthetase activity.
- Treatment with IFNs and theophylline.
- Analysis of cAMP pathway activation.
Main Results:
- Basal 2-5A synthetase activity was higher in IFN-resistant cell lines compared to susceptible ones.
- IFN treatment increased 2-5A synthetase activity in susceptible cells but not resistant cells.
- Theophylline induced 2-5A synthetase only in the L929 cell line.
Conclusions:
- IFN-resistant cells exhibit elevated basal 2-5A synthetase levels.
- A common mechanism may explain the lack of induction by both IFN and theophylline in resistant cells.
- Further research is needed to elucidate the specific molecular pathways involved in IFN resistance and 2-5A synthetase regulation.