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Translocated c-myc genes produce chimeric transcripts containing antisense sequences of the immunoglobulin heavy
M A Julius1, A J Street, P D Fahrlander
1Department of Biochemistry, State University of New York, Stony Brook 11794-5215.
Abstract:
Immunoglobulin heavy chain gene antisense transcripts contribute to the expression of translocated c-myc genes in several murine plasma cell tumors. These novel, chimeric transcripts comprise 5-50% of steady-state c-myc mRNA. Two transcripts isolated as cDNA clones use the normal splice donor and acceptor sites within the c-myc first intron. Another cDNA clone has the potential for encoding two types of c-myc proteins. The significance of immunoglobulin heavy chain gene antisense transcripts and transcriptional competence of the immunoglobulin heavy chain locus for c-myc expression is discussed.
Insights
Immunoglobulin heavy chain antisense transcripts drive c-myc gene expression in murine plasma cell tumors. These novel transcripts are key to understanding c-myc gene regulation and tumor development.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Translocated c-myc genes are implicated in murine plasma cell tumors.
- The role of immunoglobulin heavy chain (Igh) gene antisense transcripts in this process is not fully understood.
Purpose of the Study:
- To investigate the contribution of Igh gene antisense transcripts to the expression of translocated c-myc genes in murine plasma cell tumors.
- To characterize the nature and potential function of these novel chimeric transcripts.
Main Methods:
- Isolation and characterization of complementary DNA (cDNA) clones representing the chimeric transcripts.
- Analysis of splice donor and acceptor sites within the c-myc first intron.
- Assessment of the potential for encoding different c-myc protein isoforms.
Main Results:
- Novel, chimeric transcripts containing Igh antisense sequences were identified, comprising 5-50% of steady-state c-myc messenger RNA (mRNA).
- Two characterized cDNA clones utilized normal splice sites within the c-myc first intron.
- One cDNA clone indicated the potential for encoding two distinct c-myc protein types.
Conclusions:
- Igh gene antisense transcripts play a significant role in the expression of translocated c-myc genes in murine plasma cell tumors.
- The transcriptional competence of the Igh locus contributes to c-myc expression, offering insights into oncogenesis.