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Related Experiment Videos

The disc angiogenesis system.

L F Fajardo1, J Kowalski, H H Kwan

  • 1Department of Pathology, Stanford University School of Medicine, Palo Alto, California.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|June 1, 1988
PubMed
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A novel disc angiogenesis system allows in vivo study of blood vessel growth. This inexpensive and reproducible method quantifies vessel and stromal proliferation, aiding research into angiogenesis and its inhibitors.

Area of Science:

  • Biomedical Engineering
  • Vascular Biology
  • Drug Discovery

Background:

  • Studying angiogenesis (new blood vessel formation) in vivo is crucial for understanding development, wound healing, and diseases like cancer.
  • Existing methods for in vivo angiogenesis research can be complex and lack quantitative measures.
  • A simplified, reproducible system is needed to investigate angiogenic factors and inhibitors.

Purpose of the Study:

  • To develop and validate a novel, inexpensive, and reproducible in vivo system for studying angiogenesis.
  • To enable quantitative assessment of blood vessel and stromal cell proliferation into a porous scaffold.
  • To demonstrate the system's utility in evaluating angiogenic stimulants and inhibitors.

Main Methods:

  • A polyvinyl alcohol foam disc, filtered on flat sides, was used as a scaffold for cell penetration.

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  • Angiogenic agents or inhibitors were incorporated into the disc's center for slow release.
  • Discs were implanted subcutaneously in mice, retrieved at optimal time points (7-20 days), and analyzed via stained tissue sections using morphometric techniques.

    • Main Results:

    • The system successfully facilitated centripetal growth of vessels and stroma into the disc.
    • Quantitative measurements of this growth were achieved using simple morphometric techniques.
    • The system was used to study the effects of epidermal growth factor and acidic fibroblast growth factor, and to demonstrate hyperthermia-induced inhibition of vessel growth.

    Conclusions:

    • The disc angiogenesis system is a simple, inexpensive, and well-tolerated method for in vivo angiogenesis research.
    • Its reproducibility and ease of use make it suitable for a wide range of applications in vascular biology and drug discovery.
    • This system provides a valuable tool for quantifying angiogenesis and evaluating the efficacy of pro-angiogenic and anti-angiogenic agents.