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Three types of vitronectin in human blood
K Kubota1, S Katayama, M Matsuda
1Department of Biology, Ochanomizu University, Tokyo, Japan.
Cell Structure and Function
|April 1, 1988
Summary
Human blood contains vitronectin, a glycoprotein, in three distinct types based on its polypeptide composition. These vitronectin types do not correlate with age, sex, or blood type.
Area of Science:
- Biochemistry
- Molecular Biology
- Human Physiology
Background:
- Vitronectin is a cell-adhesive glycoprotein found in human serum and plasma.
- Also known as serum spreading factor and complement S-protein.
- Vitronectin exists as a mixture of a 75 kDa polypeptide and its nicked 65 kDa plus 10 kDa product.
Purpose of the Study:
- To classify human blood samples based on vitronectin composition.
- To determine the prevalence of different vitronectin types in the population.
- To investigate potential correlations between vitronectin type and demographic factors.
Main Methods:
- Quantitative immunoblotting assay was used to analyze human blood samples.
- Samples were classified into three distinct vitronectin types based on the ratio of 75 kDa to 65 kDa polypeptides.
Main Results:
- Three vitronectin types were identified: Type I (58% of population) was 75 kDa rich, Type II (35%) had equal amounts of 75 kDa and 65 kDa, and Type III (5%) was 65 kDa rich.
- No correlation was found between vitronectin type and age, sex, or ABO blood type.
Conclusions:
- Human populations exhibit distinct vitronectin composition types.
- These variations in vitronectin polypeptide ratios are independent of common demographic factors.