[Study of the expression and function of Syk and VEGF-C in the development of non-small cell lung cancer]
Jia-zhong Guo1, Qi-feng Sun1, Bo Cong1
1Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China.
Objective:
To explore the role of Syk and VEGF-C in the development of NSCLC.
Methods:
Transfered these genes(eukaryotic expression vector pcDNA3.1-VEGF-C and pLNCX-syk)into A549 cells with the liposomes. Tested the expression of VEGF-C mRNA and Syk mRNA with RT-PCR.Investigated the cell invasion assay with transwell chamber in vitro. Analysis the expression of VEGF-C proteins in A549 cells and detect Syk and VEGF-C proteins of 81 NSCLC tissue samples with immunohistochemical staining.
Results:
Higher expression of VEGF-C was revealed in VEGF-C-construct-transfected A549 cell than that in controls through RT-PCR (P < 0.05) and immunohistochemistry(P < 0.01).RT-PCR also revealed that Syk expression was higher in Syk-construct-transfected cells than in controls (P < 0.05). The cell invading experiments showed that there was more invaded cells in both transfected terms than in controls (P < 0.05). The expression of Syk protein in NSCLC tissue were significantly lower than that in the normal lung tissue (P < 0.05), while the expression of VEGF-C protein in NSCLC tissue were significantly higher than that in the normal lung tissue(P < 0.05). The expression of Syk and VEGF-C has a negative correlationship (r = -1.000, P = 0.019).
Conclusion:
The expression of Syk and VEGF-C has a negative correlationship in NSCLC tissue, VEGF-C-construct-transfected A549 cells are more aggressive than Syk-construct-transfected cells. And they may cooperated with each other in the development of NSCLC.
Insights
Spleen tyrosine kinase (Syk) and vascular endothelial growth factor C (VEGF-C) show a negative correlation in non-small cell lung cancer (NSCLC) tissues. VEGF-C promotes NSCLC cell invasion, suggesting a role in tumor development.
Area of Science:
- Oncology
- Molecular Biology
Background:
- Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality.
- Understanding the molecular mechanisms driving NSCLC progression is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the roles of Spleen tyrosine kinase (Syk) and Vascular Endothelial Growth Factor C (VEGF-C) in NSCLC development.
- To explore the relationship between Syk and VEGF-C expression in NSCLC.
Main Methods:
- Gene transfection of A549 cells with VEGF-C and Syk constructs.
- Quantitative analysis of gene and protein expression using RT-PCR and immunohistochemistry.
- In vitro cell invasion assays.
- Correlation analysis of Syk and VEGF-C expression in NSCLC tissues.
Main Results:
- VEGF-C and Syk expression were significantly upregulated in transfected A549 cells compared to controls.
- Both VEGF-C and Syk overexpression increased NSCLC cell invasion.
- Syk protein expression was lower in NSCLC tissues than in normal lung tissues, while VEGF-C expression was higher.
- A significant negative correlation was observed between Syk and VEGF-C expression in NSCLC tissues.
Conclusions:
- Syk and VEGF-C exhibit a negative correlation in NSCLC.
- VEGF-C appears to enhance the aggressiveness of NSCLC cells.
- These findings suggest a potential cooperative role for Syk and VEGF-C in NSCLC pathogenesis.
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