Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pathophysiology of Heart Failure01:17

Pathophysiology of Heart Failure

4.8K
Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...
4.8K
Heart Failure II: Pathophysiology01:29

Heart Failure II: Pathophysiology

1.9K
Systolic Heart Failure and Compensatory MechanismsSystolic heart failure (also termed HFrEF, Heart Failure with Reduced Ejection Fraction) is the most prevalent type of heart filure. It results in a decreased volume of blood being pumped from the ventricle. The aortic arch and carotid sinuses have baroreceptors that detect reduced blood pressure, triggering the sympathetic nervous system (SNS) to release epinephrine and norepinephrine. Initially, this response aims to boost heart rate and...
1.9K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

8.8K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
8.8K
Mitochondrial Membranes01:45

Mitochondrial Membranes

11.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
11.7K
Ischemic Heart Disease: Overview01:17

Ischemic Heart Disease: Overview

3.8K
Ischemic heart disease occurs when the heart's blood supply dwindles, causing an ominous lack of oxygen and nutrients. This deficiency, stemming from reduced or obstructed blood flow, spells danger, leading to heart muscle damage and dysfunction.
Atherosclerosis, the primary malefactor, orchestrates this dangerous condition. It manifests as the accumulation of fatty deposits, akin to insidious plaques, within arterial walls. As time elapses, these plaques metamorphose, hardening and...
3.8K
Myocarditis I: Introduction01:21

Myocarditis I: Introduction

645
Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...
645

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Persistent Thirst and Its Clinical Correlates in Critically Ill Adults: A Prospective Observational Study.

Nursing in critical care·2026
Same author

Acute in vivo proximity labeling for membrane targeted proteomics in neuronal circuits.

bioRxiv : the preprint server for biology·2026
Same author

EpCAM<sup>+</sup> Extracellular Vesicle PD-L1 Dynamics as a Predictive Biomarker of Immune Checkpoint Blockade Response.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Multivitamin supplementation and COVID-19 incidence and symptom severity in the COcoa Supplement and Multivitamin Outcomes Study (COSMOS) randomized trial.

The American journal of clinical nutrition·2026
Same author

Unmet Healthcare Needs and Associated Factors Among Older Adults with Osteoporosis: A Cross-Sectional Analysis of the 2025 Korea Community Health Survey.

Healthcare (Basel, Switzerland)·2026
Same author

Multimodal classification of neurons in the lateral septum.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 2, 2026

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

13.8K

Mitochondrial proteome remodeling in ischemic heart failure.

Tingting Liu1, Le Chen1, Eunjung Kim2

  • 1Molecular & Cellular Cardiology, Cardiovascular Division, University of California - Davis, Davis, CA, USA.

Life Sciences
|February 20, 2014
PubMed
Summary

This study reveals specific mitochondrial protein changes in ischemic heart failure (HF). These alterations correlate with impaired mitochondrial function, offering new insights into HF pathology.

Keywords:
Complex IComplex IIComplex IVHeartHeart failureIschemic heart failureMitochondriaNADH dehydrogenaseNDUFA5NDUFV1Proteome remodeling

More Related Videos

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics
07:03

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics

Published on: August 23, 2024

1.5K
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
07:23

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

8.8K

Related Experiment Videos

Last Updated: May 2, 2026

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

13.8K
Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics
07:03

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics

Published on: August 23, 2024

1.5K
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
07:23

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

8.8K

Area of Science:

  • Biochemistry
  • Cardiovascular Biology
  • Mitochondrial Biology

Background:

  • Mitochondrial dysfunction contributes significantly to cardiac function decline in heart failure.
  • Understanding specific molecular changes in heart failure is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate specific abnormalities in mitochondrial function and proteome during the progression of ischemic heart failure (HF).
  • To identify differentially expressed mitochondrial proteins in ischemic HF.

Main Methods:

  • Ischemic heart failure (HF) was induced in male rats via left anterior descending artery (LAD) ligation.
  • Mitochondrial function was assessed by measuring ATP levels, respiration, and Complex I activity.
  • Left ventricle mitochondrial proteome was analyzed using mass spectrometry.

Main Results:

  • Electron microscopy revealed mitochondrial structural changes in ischemic HF, including decreased size and cristae loss.
  • Mitochondria exhibited reduced ATP production, impaired respiration, and decreased Complex I activity.
  • Mass spectrometry identified 31 differentially expressed mitochondrial proteins, with 16 down-regulated and 15 up-regulated, including several Complex I proteins.

Conclusions:

  • Specific changes in mitochondrial protein expression were identified in ischemic heart failure for the first time.
  • These proteomic alterations correlate with observed functional impairments in mitochondria.
  • Distinct proteomic profiles suggest different pathological mechanisms between ischemic HF and pressure overload-induced HF.