Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

3.4K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amuc_1100 alleviates HFD-induced hepatic lipid accumulation via gut microbiota in zebrafish: insights from the role of intestinal 14-3-3β/α-A.

Journal of animal science and biotechnology·2026
Same author

Impact of chronic single or combined exposure to bisphenol A and perfluorooctanoic acid on human osteoblast spheroid.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Sperm Imprinted Gene Methylation and DNA Fragmentation in ICSI Outcomes: A Pilot Study.

Epigenomes·2026
Same author

Microplastics as both a driver of genitourinary cancers and a deliverer of treatments.

Communications medicine·2026
Same author

Effects of Cytotoxic Therapies on the Sperm Genome and Epigenome.

Advances in experimental medicine and biology·2026
Same author

PFOA effects on osteoblast differentiation: Involvement of oxidative stress and endocannabinoid receptors.

General and comparative endocrinology·2026

Related Experiment Video

Updated: May 2, 2026

A Protocol for Laboratory Housing of Turquoise Killifish Nothobranchius furzeri
07:58

A Protocol for Laboratory Housing of Turquoise Killifish Nothobranchius furzeri

Published on: April 11, 2018

18.8K

Melatonin-mediated effects on killifish reproductive axis.

Francesco Lombardo1, Giorgia Gioacchini1, Adele Fabbrocini2

  • 1Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, Ancona, Italy.

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
|February 20, 2014
PubMed
Summary

Melatonin enhances killifish reproduction by boosting fertility and embryo development. It also improves sperm quality and acts as a maternal factor for adaptive inheritance.

Keywords:
F. heteroclitusFT-IRFecundityRT-PCRSperm Class Analyzer®Sperm motilitymtnr

More Related Videos

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
09:43

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri

Published on: April 24, 2018

7.2K
Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka Oryzias latipes
05:31

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka Oryzias latipes

Published on: December 11, 2020

4.1K

Related Experiment Videos

Last Updated: May 2, 2026

A Protocol for Laboratory Housing of Turquoise Killifish Nothobranchius furzeri
07:58

A Protocol for Laboratory Housing of Turquoise Killifish Nothobranchius furzeri

Published on: April 11, 2018

18.8K
Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
09:43

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri

Published on: April 24, 2018

7.2K
Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka Oryzias latipes
05:31

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka Oryzias latipes

Published on: December 11, 2020

4.1K

Area of Science:

  • * Reproductive biology and endocrinology
  • * Aquatic toxicology and ecotoxicology

Background:

  • * Melatonin is a key hormone regulating circadian rhythms and reproduction in vertebrates.
  • * The brackish killifish (Fundulus heteroclitus) is a valuable model for studying reproductive physiology in response to environmental cues.

Purpose of the Study:

  • * To investigate the effects of melatonin on the neuroendocrine axis and reproductive parameters in female and male killifish.
  • * To determine melatonin's role in enhancing fecundity, embryo survival, and hatching rates.
  • * To explore melatonin's influence on sperm quality and its potential as a maternal factor in offspring development.

Main Methods:

  • * Analysis of gene expression for gonadotropin-releasing hormone (GnRH), luteinizing hormone receptor (LHR), and melatonin receptor (MTNR) in female killifish.
  • * Assessment of sperm motility, velocity, and lipid unsaturation levels using Fourier Transform Infrared (FT-IR) microspectroscopy in male killifish.
  • * Evaluation of embryo and larval biometric parameters in offspring from melatonin-exposed parents.

Main Results:

  • * Melatonin significantly increased fecundity, embryo survival, and hatching rates in female killifish.
  • * Melatonin enhanced sperm motility and velocity in males, with FT-IR showing reduced lipid unsaturation in spermatozoa.
  • * Reduced MTNR expression and lower larval biometric parameters were observed in offspring from melatonin-exposed parents, suggesting maternal transfer of signals.

Conclusions:

  • * Melatonin plays a positive role in enhancing killifish reproduction, impacting both parental reproductive capacity and offspring development.
  • * Melatonin acts as a maternal factor, mediating the transfer of environmental signals to oocytes and embryos for adaptive inheritance.
  • * These findings highlight melatonin's significance in the reproductive success and adaptive potential of killifish populations.