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Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

A versatile ultra-high performance LC-MS method for lipid profiling.

Oskar L Knittelfelder¹, Bernd P Weberhofer¹, Thomas O Eichmann¹

¹Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|February 20, 2014

Summary

This summary is machine-generated.

A novel untargeted lipidomic method using UPLC and qTOF MS improves lipid separation and identification. This approach enhances the analysis of complex lipid species, including low-abundance phosphatidic acid, in biological samples like yeast.

Keywords:

Glycerophospholipids Lipidomics Mass spectrometry Neutral lipids Ultra-performance liquid chromatography

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Area of Science:

Analytical Chemistry
Biochemistry
Mass Spectrometry

Background:

Lipidomics research requires advanced analytical techniques for comprehensive profiling.
Separating and identifying diverse lipid species, especially low-abundance ones, presents significant challenges.

Purpose of the Study:

To introduce a new UPLC-based untargeted lipidomic approach for enhanced lipid analysis.
To improve the separation and identification of complex lipid species, including isomers and low-abundance classes.
To apply the method for investigating the lipidome of yeast.

Main Methods:

Utilized Ultra-Performance Liquid Chromatography (UPLC) coupled with a quadrupole time-of-flight (qTOF) hybrid mass spectrometer.
Employed a binary gradient elution with phosphoric acid additive for improved peak shape of acidic phospholipids.
Implemented MS(E) scans for simultaneous full scan data and fragmentation acquisition.

Main Results:

Achieved effective separation of lipid species, including constitutional isomers.
Enabled detection and characterization of low-abundant lipid classes like phosphatidic acid (PA).
Successfully applied the method to investigate the yeast lipidome, providing detailed lipid profiles.

Conclusions:

The developed UPLC-qTOF method offers a robust platform for untargeted lipidomics.
The approach enhances the identification and structural elucidation of lipids.
This method is valuable for comprehensive lipidome analysis in various biological systems.