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Related Experiment Videos

Signal recognition-like particles are present in maize.

N Campos1, J Palau, M Torrent

  • 1Departamento de Biologia Molecular i Agrobiologia, Centre d'Investigació i Desenvolupament Consejo Superior de Investigacions Cientificas, Jordi Girona Salgado, Barcelona, Spain.

The Journal of Biological Chemistry
|July 15, 1988
PubMed
Summary

Maize storage protein translocation involves signal recognition particles (SRPs), similar to animal systems. This study identifies a maize SRP-like factor that restores protein transport but does not arrest translation.

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Area of Science:

  • Plant molecular biology
  • Cellular and subcellular biology
  • Biochemistry

Background:

  • Protein translocation across membranes is crucial for cellular function.
  • Signal recognition particles (SRPs) mediate protein targeting in animal systems.
  • Understanding plant protein transport mechanisms is essential for crop improvement.

Purpose of the Study:

  • To investigate the role of signal recognition particles (SRPs) in maize storage protein translocation.
  • To characterize the SRP-like factor in maize endosperm.
  • To compare the function of maize SRP with canine SRP.

Main Methods:

  • Preparation of a high-salt extract from maize endosperm cell homogenates.
  • Isolation of a ribosome-free fraction enriched in SRP-like activity.

Related Experiment Videos

  • Assay of translocation competence using salt-washed maize microsomes and pre-zein mRNA.
  • Electrophoretic and sedimentation analysis of the SRP-like particle and its RNA component.

    • Main Results:

    • A maize SRP-like factor, a 12 S ribonucleoprotein particle, was identified in endosperm extracts.
    • The RNA component of the maize SRP-like particle is complex, with a three-band electrophoretic pattern and 8 S sedimentation.
    • This maize SRP-like fraction restored translocation competence to salt-washed microsomes.
    • Unlike canine SRPs, the maize component did not arrest zein protein translation in a wheat germ system.

    Conclusions:

    • Maize storage protein translocation across microsomal membranes is mediated by SRPs, analogous to animal systems.
    • The identified maize SRP-like factor plays a role in targeting storage proteins to the endoplasmic reticulum.
    • Functional differences exist between plant and animal SRPs regarding translation arrest activity.