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Published on: March 20, 2016
Engineered riboswitch as a gene-regulatory platform for reducing antibiotic resistance
1Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, People's Republic of China.
Abstract:
Antibiotic resistance (AR), the ability of a microorganism to withstand the effects of antibiotics, is a growing and increasingly serious global public health problem. Enzymatic activation of antibiotics though the production of β-lactamase is one of the main mechanisms causing AR. Synthetic riboswitch containing aptazyme is constructed in E. coli to regulate the expression of β-lactamase through small molecule-aptamer interactions, which sharply reduces the antibiotic resistance of the engineered bacteria.
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