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Updated: May 2, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
[Enzymes of carbohydrate metabolism in nectaries]
M A de Fekete1, H Ziegler, R Wolf
1Botanisches Institut der Technischen Hochschule Darmstadt, Darmstadt, Deutschland.
Abstract:
1. The activity of some important enzymes of carbohydrate metabolism was investigated in several fractions of homogenized tissues of nectaries from Deutzia scabra and Convolvulus sepium in the secretion-phase. Considerable activity was found for the particle-associated hexokinase and the soluble fructokinase, phosphohexoseisomerase, sucrase, UTP-glucose-1-phosphate-uridyltransferase, acid and alkaline phosphatases, and pyrophosphatase. The activity of phosphoglucomutase was smaller, and that of starch-hydrolases and-phosphorylases and of sucrose-synthetase was very weak. 2. The meaning of these results in relation to the metabolism and the secretion of carbohydrates in nectaries is discussed.
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