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Updated: May 2, 2026

Microtiter Dish Biofilm Formation Assay
Published on: January 30, 2011
cAMP signaling affects irreversible attachment during biofilm formation by Pseudomonas aeruginosa PAO1
Kaori Ono1, Rie Oka, Masanori Toyofuku
1Graduate School of Life and Environmental Sciences, University of Tsukuba.
Abstract:
Pseudomonas aeruginosa responds to environmental changes and regulates its life cycle from planktonic to biofilm modes of growth. The control of cell attachment to surfaces is one of the critical processes that determine this transition. Environmental signals are typically relayed to the cytoplasm by second messenger systems. We here demonstrated that the second messenger, cAMP, regulated the attachment of cells. Our results suggest cAMP inhibited the transition from reversible to irreversible attachment. Further analyses revealed that cell surface hydrophobicity, one of the key factors in cell attachment, was altered by cAMP.
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