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Circulating progenitor cells in hypertensive patients with different degrees of cardiovascular involvement

G Mandraffino1, E Imbalzano1, M A Sardo1

  • 1Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy.

Journal of Human Hypertension
|February 21, 2014
PubMed

Insights

Hypertension impacts proangiogenic hematopoietic cells (PHCs) differently based on cardiovascular involvement. Arterial stiffening increases PHCs with oxidative stress, while left ventricular hypertrophy reduces them.

Area of Science:

  • Cardiovascular Biology
  • Hematology
  • Oxidative Stress Research

Background:

  • Hypertension is linked to cardiovascular damage and altered circulating cell populations.
  • Proangiogenic hematopoietic cells (PHCs) play a role in vascular health, but their behavior in different hypertension stages is unclear.
  • The redox system and microRNA expression within PHCs may be affected by hypertensive cardiovascular involvement.

Purpose of the Study:

  • To investigate the association between hypertension-related cardiovascular involvement (arterial stiffening vs. left ventricular hypertrophy) and PHC numbers, phenotypes, and redox status.
  • To evaluate microRNA (miRs) 221/222 expression in CD34+ cells and its relationship with cell number, reactive oxygen species (ROS), and key redox enzymes.
  • To compare these parameters between isolated arterial stiffening (AS) hypertensives and those with left ventricular hypertrophy (LVH).

Main Methods:

  • Isolation of PHCs from hypertensive patients and controls using flow cytometry.
  • Analysis of CD34+ cell expression of miRs 221/222, ROS levels, and redox enzymes (MnSOD, CAT, GPx-1, NOX2).
  • Comparison of PHC numbers and redox system components across different hypertensive subgroups (AS, LVH) and controls.

Main Results:

  • PHCs were elevated in hypertensives compared to controls but lower in LVH than in AS hypertensives.
  • In AS hypertensives, CD34+ cells showed overexpression of NOX2, MnSOD, CAT, and GPx-1, with increased ROS and miRs associated with cell number.
  • In LVH, an imbalanced redox system (high MnSOD, low CAT/GPx-1) was observed, with higher ROS and miRs inversely associated with cell number.

Conclusions:

  • In AS hypertensives, a balanced redox system may support increased PHCs.
  • In hypertensives with more advanced lesions (LVH), redox imbalance likely leads to increased oxidative stress and reduced PHCs.
  • These findings highlight distinct PHC responses and redox alterations in different stages of hypertensive cardiovascular involvement.

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