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Updated: Aug 19, 2026

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A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy
Published on: April 30, 2015
Light microscopical demonstration and zonal distribution of parasinusoidal cells (Ito cells) in normal human liver
1Department of Pathology, Hvidovre Hospital, University of Copenhagen, Denmark.
Abstract:
The parasinusoidal cells of the liver (Ito cells) were demonstrated light microscopically in autopsy specimens fixed in formalin and stained with Oil red O after dichromate treatment. The method allows examination of large samples containing numerous acini. Quantitative assessment showed a zonal gradient with 6.3 and 7.7 parasinusoidal cells per 62.5 X 10(3) micron2 in zone 1 and 3, respectively.
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