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Isolation of ribosomes from pollen
1Marine Biological Laboratory, Fertilization and Gamete Physiology Research Program, Woods Hole, Massachusetts.
Abstract:
Ribosomes have been isolated from lily pollen. The size distribution on sucrose gradients is similar to ribosomes from vegetative plant material. Some fractions in ungerminated pollen seem to be resistant to ribonuclease. With progressing germination the sucrose gradient profiles of ribosome preparations undergo changes, indicating changes in the translation process in correlation to a developmental process.ZUSAMMENFASSUNG: Aus Pollen von Lilien konnten Ribosomen isoliert werden. Das Profil am Zuckergradienten ist ähnlich dem Polysomen-Profil, wie es für vegetatives Pflanzenmaterial beschrieben ist. In ungekeimtem Pollen erscheinen einige Fraktionen besonders stabil gegenüber Ribonuklease. Mit fortschreitender Keimung ändert sich das Profil am Zucker-Gradienten. Dies kann als Ausdruck der Änderungen im Translation-Prozeß im Zusammenhang mit einem Entwicklungsprozeß angesehen werden.
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