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Mucilaginibacter auburnensis sp. nov., isolated from a plant stem
Peter Kämpfer1, Hans-Jürgen Busse2, John A McInroy3
1Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
International Journal of Systematic and Evolutionary Microbiology
|February 21, 2014
Summary
A novel bacterium, Mucilaginibacter auburnensis, was identified as an endophyte in corn stems. This yellow-pigmented, Gram-negative bacterium represents a new species within the Mucilaginibacter genus, expanding our understanding of plant-associated microbes.
Area of Science:
- Microbiology
- Plant Pathology
- Bacteriology
Background:
- Endophytic bacteria reside within plant tissues, playing crucial roles in plant health and development.
- The genus Mucilaginibacter comprises Gram-negative bacteria, with limited characterization of its species and their ecological roles.
- Isolation of novel bacteria from plant endosphere provides insights into microbial diversity and plant-microbe interactions.
Purpose of the Study:
- To characterize a novel yellow-pigmented, Gram-negative, rod-shaped bacterium isolated from corn stems.
- To determine the phylogenetic and taxonomic position of the isolate within the genus Mucilaginibacter.
- To propose a new species designation based on comprehensive polyphasic characterization.
Main Methods:
- Isolation of strain JM-1070T from internal corn stem tissue.
- Phylogenetic analysis using 16S rRNA gene sequencing.
- DNA-DNA hybridization for genetic relatedness assessment.
- Analysis of chemotaxonomic features: major respiratory quinones, predominant fatty acids, polar lipids, and polyamine profiles.
Main Results:
- Strain JM-1070T, a yellow-pigmented, Gram-negative, rod-shaped bacterium, was identified as a presumptive endophyte.
- Phylogenetic analyses placed the strain within the genus Mucilaginibacter, closely related to M. composti and M. calamicampi, but DNA-DNA hybridization confirmed low relatedness.
- Chemotaxonomic analyses revealed characteristic menaquinones (MK-6, MK-7), specific fatty acid profiles (iso-C15:0, summed feature 3, iso-C17:0 3-OH), and sym-homospermidine as the predominant polyamine.
Conclusions:
- Strain JM-1070T represents a novel species of the genus Mucilaginibacter, distinct from previously described species.
- The proposed name for this new species is Mucilaginibacter auburnensis sp. nov., with JM-1070T as the type strain.
- This discovery contributes to the understanding of endophytic bacterial diversity in agricultural crops like corn.
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