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Clavibacter michiganensis subsp. phaseoli subsp. nov., pathogenic in bean

Ana J González1, Estefanía Trapiello1

  • 1Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Principado de Asturias, 33300 Villaviciosa, Spain.

International Journal of Systematic and Evolutionary Microbiology
|February 21, 2014
PubMed

Insights

A novel bacterium causing bacterial bean leaf yellowing was identified in bean seeds. This new pathogen, Clavibacter michiganensis subsp. phaseoli, represents a distinct subspecies affecting Phaseolus vulgaris.

Area of Science:

  • Plant Pathology
  • Bacteriology
  • Molecular Biology

Background:

  • Clavibacter michiganensis is a known plant pathogen.
  • Accurate identification of bacterial pathogens is crucial for disease management.

Purpose of the Study:

  • To identify and characterize a novel bacterial pathogen isolated from bean seeds.
  • To determine if the isolate represents a known or new subspecies of Clavibacter michiganensis.

Main Methods:

  • 16S rRNA gene sequencing for initial identification.
  • Polymerase Chain Reaction (PCR), Amplified Ribosomal DNA Restriction Analysis (ARDRA), Restriction Fragment Length Polymorphism (RFLP), and Multilocus Sequence Analysis (MLSA) for subspecies identification.
  • Biochemical and phenotypic analyses using API 50CH and API ZYM.
  • Pathogenicity tests on bean plants (Phaseolus vulgaris L.).

Main Results:

  • The isolated bacterium was identified as Clavibacter michiganensis.
  • Molecular and phenotypic analyses indicated that the strain (LPPA 982T) did not match any known subspecies.
  • Pathogenicity tests confirmed the strain causes a new disease in beans, termed bacterial bean leaf yellowing.
  • The strain was proposed as a new subspecies, Clavibacter michiganensis subsp. phaseoli subsp. nov.

Conclusions:

  • A novel subspecies of Clavibacter michiganensis, C. michiganensis subsp. phaseoli, has been identified.
  • This subspecies is a pathogen of Phaseolus vulgaris, causing bacterial bean leaf yellowing.
  • The findings contribute to understanding bacterial plant diseases and pathogen diversity.

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