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Published on: June 16, 2018
[Study on quality of five hybrid corn kernels by FTIR spectroscopy]
You-Ming Shi1, Cui-Qiong Yan2, Yan-Lin Sun2
1College of Physics and Electronic Engineering, Qujing Normal University, Qujing 655011, China. sym8295@163.com
Fourier transform infrared (FTIR) spectroscopy combined with curve-fitting offers a rapid method for analyzing corn kernel quality. This technique accurately quantifies starch and protein concentrations, aiding hybrid corn studies.
Area of Science:
- Agricultural Science
- Analytical Chemistry
- Spectroscopy
Background:
- Assessing corn kernel quality is crucial for agricultural applications.
- Traditional methods for analyzing corn composition can be time-consuming.
- Developing rapid and accurate analytical techniques is essential for hybrid corn research.
Purpose of the Study:
- To evaluate the feasibility of using Fourier transform infrared (FTIR) spectroscopy for corn kernel quality assessment.
- To investigate the potential of FTIR spectroscopy combined with curve-fitting for analyzing corn kernel composition.
- To establish a rapid and convenient method for studying hybrid corn.
Main Methods:
- Acquisition of infrared spectra from five types of hybrid corn kernels using an infrared spectrometer.
- Analysis of spectral data in the range of 1776–952 cm⁻¹.
- Application of curve-fitting techniques to resolve overlapping spectral bands of starch, protein, and lipid.
Main Results:
- The infrared spectrum primarily comprises absorption bands related to starch, protein, and lipid.
- Characteristic bands for starch, protein, and lipid showed significant overlap, obscuring detailed information.
- Curve-fitting revealed significant information, demonstrating a linear relationship between band area percentages (at 1051 and 1548 cm⁻¹) and the concentrations of starch and protein.
Conclusions:
- Fourier transform infrared spectroscopy, when coupled with curve-fitting, is a feasible method for analyzing starch and protein concentrations in corn kernels.
- This combined approach provides a rapid and convenient tool for the quality assessment and study of hybrid corn.
- The method offers a valuable alternative to traditional, more time-intensive analytical techniques.
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